Applied and Environmental Microbiology (1995) 61, 966-971

T.B. Adhikari, C.M.V. Cruz, Q. Zhang, R.J. Nelson, D.Z. Skinner, T.W. Mew and J.E. Leach (1995)
Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia
Applied and Environmental Microbiology 61 (3), 966-971
Abstract: Restriction fragment length polymorphism and virulence analyses were used to evaluate the population structure of Xanthomonas oryzae pv. oryzae, the rice bacterial blight pathogen, from several rice-growing countries in Asia. Two DNA sequences from X. oryzae pv. oryzae, IS1112, an insertion sequence, and avrXa10, a member of a family of avirulence genes, were used as probes to analyze the genomes of 308 strains of X. oryzae pv. oryzae collected from China, India, Indonesia, Korea, Malaysia, Nepal, and the Philippines. On the basis of the consensus of three clustering statistics, the collection formed five clusters. Genetic distances within the five clusters ranged from 0.16 to 0.51, and distances between clusters ranged from 0.48 to 0.64. Three of the five clusters consisted of strains from a single country. Strains within two clusters, however, were found in more than one country, suggesting patterns of movement of the pathogen. The pathotype of X. oryzae pv. oryzae was determined for 226 strains by inoculating five rice differential cultivars. More than one pathotype was associated with each cluster; however, some pathotypes were associated with only one cluster. Most strains from South Asia (Nepal and India) were virulent to cultivars containing the bacterial blight resistance gene xa-5, while most strains from other countries were avirulent to xa-5. The regional differentiation of clusters of X. oryzae pv. oryzae in Asia and the association of some pathotypes of X. oryzae pv. oryzae with single clusters suggested that strategies that target regional resistance breeding and gene deployment are feasible.
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Database assignments for author(s): Rebecca J. Nelson, Jan E. Leach, Tika B. Adhikari

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas oryzae		Indonesia
	Xanthomonas oryzae		Korea-South
	Xanthomonas oryzae		Malaysia
	Xanthomonas oryzae		Philippines
	Xanthomonas oryzae		India
	Xanthomonas oryzae		Nepal